yellowjackets | Ibycter

This is a post to announce my new paper, which has a bit of a mouthful for a title: Developing a paired-target apparatus for quantitative testing of nest defense behavior by vespine wasps in response to con- or heterospecific nest defense pheromones

This is a paper that was long in the making, and there are two important things you should take away from it: 1) the bioassay setup is cool and 2) three species of the genus Vespula recognize each other’s alarm pheromones

OK, so why alarm pheromones? Well, many social insects coordinate their defences against predators using chemical signalling. These signals can arouse a colony into defensive behaviour, and often can be applied to attackers to attract other workers to attack the intruder. In some cases these chemicals are one and the same, in others, they may be different. No matter! Although our efforts here were part of an attempt to describe the alarm pheromone chemistry of these yellowjackets, we never did succeed in isolating the exact chemicals needed to mimic the naturally-occurring alarm pheromones.

Vespula pensylvanica, the western yellowjacket

Vespula alascensis, the common yellowjacket

Vespula germanica, the German yellowjacket

Many wasp species have their alarm pheromones in their venom sac, and presumably, when disturbed extrude a little venom to signal other workers, or deposit venom on nest predators to “mark” them for attack by the colony. We worked with three species of yellowjackets, Vespula pensylvanica, Vespula alascensis and Vespula germanica. The substances we tested in this study were venom sac extracts of these wasps dissolved in acetonitrile, an easy-to-work with polar solvent.

We dissected out venom sacs (the translucent jelly-bean looking thing) and crushed them in acetonitrile, at a rate of 1 sac per 10 microliters. We filtered the extracts and stored them cold for later use.

To test the effect of the putative alarm substances in these extracts, we needed a bioassay device. Because no standard protocol existed, I designed one that I hope will become the standard for any future experiments:

The paired-target bioassay

In 1995, Visscher and Vetter (yes, that Vetter! The spider guy!) developed a resonant-target attack counter for use in quantifying defensive attacks by yellowjackets and bumblebees. It so happens that when these flying stingmeisters attack, they fly at high speed toward their victim and strike it with force. Visscher and Vetter’s device utilized this important fact by surrounding an audio microphone with a resonant plastic target, and using an electrical counter to count the hits. The device requires construction of the counting circuit, but I thought, why not just record an audio file and count the strikes later?

Basically the device I designed is an elaboration of Visscher and Vetter’s strike counter, only it can be assembled with off-the-shelf components, and utilizes free software to count the strikes recorded to an audio file. In addition, my design is explicitly for use in paired assays, as wasp attack behaviour is often quite variable from one situation to the next, and experiments to test the effect of alarm pheromones can benefit greatly by pairing the treatment and control in each replicate. Think of it as blocking! at 9 am, the wasps are really feisty, and hundreds come out to strike, at 10:30, the attack is less fierce. If we did a control run with a single target at 9, and a treatment target at 10:30, we would be misled, but with a paired design we can determine that our treatment has an effect in both replicates. This saves immensely on the number of replicates required to see a difference.

OK, so here is how the device looks:

The vast majority of the components are very easy to acquire, and the only construction requires a bit of drilling and screwing in of wingnuts and bolts. Everything else is off-the-shelf, and the device folds up neatly for transport and storage. The targets are formed from thin black polystyrene weigh boats, which make a drum-like sound when struck, and they can be disposed of between each replicate so that residual alarm pheromone is not a problem.

Onour Moeri bringing the paired target device to the wasps.

Here is what it looks like when a wasp strikes a target:

The basic process of doing a bioassay is to place the device so that the targets are equidistant from the nest entrance, apply a test substance to one target, a control solvent to the other, start the recording, and then tap the nest entrance to get the wasps activated (note, this step not ALWAYS needed! Some nests are on high alert anyway!)

Here is a video of the device in operation, with audio derived directly from the paired microphones. In this case, the pheromone extract is placed on the left-hand side of the device.

After each assay, the device is removed, the targets replaced, and then the treatment and control are re-applied for another replicate. I always alternated the left-right placement from one replicate to the next, to avoid any side bias.

After a number of replicates (at least 10, better 15 to 20), the audio files are offloaded from the digital recorder, and split into treatment and control files for each replicate. You can use Audacity (a freeware program) to do this.

A simple oscillogram of each file often shows an effect: This is how the two oscillograms look for the left (top) and right (bottom) channels. As you can see, the left hand side experienced more strikes in this short clip.

But how to count these strikes effectively? Well, the solution is another freeware program, SoundRuler, developed by Marcos Gridi-Papp for his teaching in bioacoustics. My application of this software is really wasting its potential, but it works quite well for counting these loud percussive strikes!

In the SoundRuler interface, some simple rules for counting the strikes are programmed in, then automated counting of the entire range desired is simple:

The SoundRuler interface with an open audio file of 240 seconds.

The same file after automated counting enabled. In this case, 123 strikes were counted.

Alright! So that is the development of the paired-target bioassay device, as well as its operation. What did we use it for?

Well, we tested whether three species of ground nesting yellowjackets, Vespula pensylvanica, Vespula alascensis and Vespula germanica have an alarm pheromone in their venom sacs. We also tested whether or not these species would recognize the alarm pheromones of the other species. It turns out that they do!

So what would be the advantage of responding to the alarm pheromone of another yellowjacket species? Well, each of these species has a very similar life history, being ground-nesting, and needing to defend the nest from the same kinds of predators (bears, skunks, raccoons, humans). I can see the advantage of responding early to a “pheromone-marked” predator which had just attacked another species, stinging it and driving it away before it has the chance to attack your own nest. These alarm pheromones probably evolved once in the vulgaris species group, and there was no selection for differentiation, and possibly selection against it.

So there you have it! An inexpensive, easy to construct bioassay device that you can use to test alarm pheromones in large stinging wasps! A counting protocol that is easy and fast! What more could you want? Well, I suggest that the first thing you could wish for is very good luck and/or insight into the complicated chemistry of these alarm substances, but this study has at least provided some new tools to get there.

OK! So that is the paper. I hope you enjoyed it, and be sure to read the whole thing over at the journal website! In the meantime, please enjoy these awesome pictures of the bioassay device in operation.

Commonly seen wasps of urban BC

Wasps are a big part of outdoor life this time of year in Coastal BC. It seems we have more than our fair share of scavenging workers pestering us at barbecues and picnics. The long dry summer was ideal for nest success and thus we have a large population approaching the end of their colony cycles. But which insects are responsible? It turns out only three of the common wasps we see are much of a nuisance outdoors, and all belong to the genus Vespula. Below are some short summaries of what they look like and what the differences are.

Genus Vespula: ground or structure nesting, large colony sizes, often scavenge in late season at outdoor cooking or dining areas. They differ from a related genus Dolichovespula by having the mandible closer to the eye than the latter.

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Nest entrance typical of Vespula wasps. They will also nest in cavities and structures

Western Yellowjacket: Vespula pensylvanica: native species, builds nests underground, has a “complete yellow eye ring” (see photo)

Common Yellowjacket: Vespula alascensis: somewhat smaller than the Western, this species has an interrupted yellow band behind the eye. This individual is gathering paper fibre for nest-building.

German Yellowjacket (Vespula germanica): This also does not have a complete band around the eye, but it does not have the interruption in the yellow band behind the eye. Often black spots are visible on the abdomen.

Dolochovespula: We have two common species in BC, the Common Aerial Yellowjacket, Dolichovespula arenaria and the Bald-faced Hornet Dolichovespula maculata. Unlike the Vespula, these hang their nests from branches and projections, and so they are often visible. They do not scavenge, except for sugar sources (you may see them on fruits or flowers).

Bald-faced Hornet nectaring.

This is a nest of Bald-faced Hornets. Aerial yellowjacket nests are similar, hanging from branches or obstructions.

Common aerial yellowjackets on their nest. The upper one is the queen, the lower one a worker.

“Paper wasps” subfamily Polistinae

Two genera are found in BC; Polistes and Mischocyttarus. They are superficially similar, but Mischocyttarus has an elongate “petiole” joining the abdomen to the thorax. Their nests are open, meaning they are without an envelope, and are often found under overhangs. They are not very aggressive.

The European Paper Wasp: Polistes dominula. This species is overall yellow and black, with yellow or orange antennae.

Polistes aurifer. This species is larger, darker, and much less common than it used to be, perhaps due to competition from Polistes dominula.

Mishchocyttarus flavitarsus. This odd paper wasp is a member of a genus that is mostly found in the tropical and subtropical parts of the Americas. This is one that makes it as far as Canada.

Summary: There is quite a diversity of social wasps in BC, many of which will not be found in cities at all. They are important players in ecosysyems, as predators, scavengers, and possibly pollinators. Avoiding conflict with them is easy, provided they don’t nest near your entrances or walkways, as stinging typically occurs during nest defense. If you wear long sleeved white or light coloured clothing with close fitting cuffs, the chance of an accidental sting are minimized. Some stings can be life-threatening to some people, so if you work with groups of people, familiarize yourself with the symptoms and treatment of anaphylaxis, and always have an Epipen and phone ready.

Nest destruction should be contemplated if the nest is going to bring people or pets very close to the nest entrance, as the number of stings from a defensive colony at close range can be severe. Over-the counter wasp killers work well for simple to access nests, but more difficult to reach nests inside structures may require the pros.

I simply leave nests be if they are out of the way and not likely to pose a threat.

If you find a nest and would like to help us out in the lab, we would love to collect some or all of the wasps! You can emal Sebastian Ibarra at [email protected] or call us at the lab at 778-782-5939.

Sebastian collecting Western Yellowjackets by the water.

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Tag Archive | yellowjackets

Counting wasp attacks: investigating alarm pheromones in yellowjackets

The paired-target bioassay

Common Social Wasps of BC